Biology Microscope

More Media in Cell Culture

admin — Thu, 05 Jul 2007 17:41:04 +0000

In the preparation of media, it is important that the time and temperature of storage of stock solutions and finished media be standardized and adhered to carefully. Many minor variations can be tolerated by cells growing in a mixture of synthetic medium and serum or other biological or proteinaceous supplements. When cells are grown entirely in synthetic media, very minor variants can be critical.

It cannot be overemphasized that all procedures, sources of reagents, and others should be standardized, empirically if need be, and adhered to rigorously.

(more…)

Surveillance Procedure Applied to Sera

admin — Thu, 05 Jul 2007 17:40:15 +0000

Here is a comparison between commercial fetal bovine serum and some special fetal bovine serum produced under conditions of maximal sterility, freedom from whole cells, and rapid processing in the cold. With the aid of a biology microscope, it has been found out that there is an improved cell growth-supporting capacity in the special fetal bovine sera than the commercial type. An approximate 10% of the commercial samples were contaminated with bacteria, fungi, or bovine viruses.

(more…)

More Preparatoin Procedures

admin — Thu, 05 Jul 2007 17:39:31 +0000

Laminar Flow System

Before using the biology microscope, prevention of airborne contamination while manipulating cultures must be observed. The transfer area is ventilated by continuous piston-like displacement of air that has been passed through a high efficiency particulate air or HEPA filter. These filters are 99.97% efficient in removal of particles 0.3um in diameter and for practical purposes they sterilize the ambient air of the transfer room.

(more…)

Glassware Preparations

admin — Thu, 05 Jul 2007 17:38:14 +0000

Disposable polystyrene plastic and soda glass containers are in common use for cell cultures, which may be viewed under a biology microscope, having both the advantage of low initial cost and can therefore be discarded after one use. Few people are required for washing and reprocessing of tissue culture vessels. Tissue culture vessels are not part of the biology microscope.

(more…)

Tissue Culture

admin — Thu, 05 Jul 2007 17:37:38 +0000

Tissue culture is the rearing up or cultivation of cells or tissues in certain media, which can be viewed under a biology microscope. It involves a series of processes and its successful results can be used in a wide variety of scientific fields. Tissue culture has developed methods particularly those involving some degree of automation. Representative protocols for the applications of these techniques were also drafted.

(more…)